Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ABD??LKADİR KARADAĞ
Date created: 2013-06-13 11:00:00
Date modified: 2015-05-08 01:13:51
RFP Generator with ROSE thermometer
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K993000_sequence (Version 1) |
Description
The repression of heat shock gene expression (ROSE) element is an RNA element found in the 5' UTR of some heat shock protein's mRNAs. The ROSE element is an RNA thermometer that negatively regulates heat shock gene expression. The secondary structure is thought to be altered by temperature, thus it is an RNA thermometer. This structure blocks access to the ribosome binding site at normal temperatures. During heat shock however, the structure changes freeing the ribosome binding site and allowing expression to occur.RNA Thermometers are the only known single-component regulators of gene expression.(1) RNA Thermometers are thermosensors that regulate gene expression by temperature-induced changes in RNA conformation. Naturally occurring RNA Thermometers exhibit complex secondary structures which are believed to undergo a series of gradual structural changes in response to temperature shifts.(2) RNA Thermometers often regulate genes required during either a heat shock or cold shock response, but have been implicated in other regulatory roles such as in pathogenecity and starvation.(3)
Notes
Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.Source
Bradyrhizobium japonicum USDA 110 and Registry| Sequence Annotation | Location | Component / Role(s) |
| pVeg2 ROSE Thermometer RFP Generator Finish Codon Start Codon | 1,97 98,193 194,902 900,902 194,196 | promoter feature/promoter ribosome_entry_site feature/rbs feature/protein CDS stop_codon feature/stop feature/start start_codon |