Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: İBRAHİM YASİR ORHAN
Date created: 2013-06-13 11:00:00
Date modified: 2015-05-08 01:13:51
IbpB RNA Thermometer with RFP
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K993002_sequence (Version 1) |
Description
The IbpB thermometer is an RNA thermometer element found in the ibpAB operon. The operon contains two heat-shock genes, encoding inclusion body binding proteins A and B (IbpA/B), and is the most drastically upregulated operon under heat-shock in Escherichia coli.IbpA is regulated by a ROSE element found in its 5' UTR, while IbpB has its own heat-sensitive cis-regulatory element. The activity of this thermoregulator was confirmed in vitro but was not found in vivo, suggesting more complicated operon regulation exists in bacterial cells. In this part, red fluorescent protein (RFP) is added in the downstream region to measure the activity of RNA thermometer. You check our Experience page for further information about our characterization and experiments. For the inner elements in the part design; check out the Part Design page.
RNA Thermometers are the only known single-component regulators of gene expression.(1) RNA Thermometers are thermosensors that regulate gene expression by temperature-induced changes in RNA conformation. Naturally occurring RNA Thermometers exhibit complex secondary structures which are believed to undergo a series of gradual structural changes in response to temperature shifts.(2) RNA Thermometers often regulate genes required during either a heat shock or cold shock response, but have been implicated in other regulatory roles such as in pathogenecity and starvation.(3)
Notes
Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.Source
Escherichia coli, NCBI GeneBankOther elements are obtained from Registry.
| Sequence Annotation | Location | Component / Role(s) |
| pVeg2 IbpB thermometer RFP Stop codon Start codon | 1,97 98,208 209,917 915,917 209,211 | promoter feature/promoter feature/rbs ribosome_entry_site CDS feature/protein stop_codon feature/stop feature/start start_codon |