Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vaibhavi Umesh
Date created: 2009-05-09 11:00:00
Date modified: 2015-05-08 01:13:51
{˂
eCPX!˃
} - circularly permuted OmpX
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_M10014_sequence (Version 1) |
Description
What it does - give refernecesThis part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
BglBricks Standard Description Page
Notes
No prepro sequenceSOE-ing PCR used
Source
1) Native C-terminal portion of ompX {N.ompX}PCR Ovu015/Ovu016 on MG1655 gen. (322bp, EcoRI/BamHI)
Sub into pBca9145-Bca1144#5 (EcoRI/BamHI, L)
Product is pBca9145-M10012 {N.cpx}
Ovu015 Construction of OmpX N term part
ctagaGAATTCatgAGATCTGGTCAGTCTggtgactacaacaaaaaccag
Ovu016 Construction of OmpX N term part
gacaaGGATCCgaagcggtaaccaacagaggcaatccaggtgcctac
2) Native N-terminal portion of ompX {C.ompX}
PCR Ovu017/Ovu018 on MG1655 gen. (196bp, EcoRI/BamHI)
Sub into pBca9145-Bca1144#5 (EcoRI/BamHI, L)
Product is pBca9145-M10013 {C.cpx}
Ovu017 Construction of OmpX C term part
ctagaGAATTCatgAGATCTgcgacttctactgtaactgg
Ovu018 Construction of OmpX C term part
gacaaGGATCCttaagagcttgcagtacggcttttctcgg
3) SOEing assembly of eCPX
PCR Ovu015/Ovu019 on pBca9145-M10012 (334bp, EcoRI/BamHI = A)
PCR Ovu020/Ovu018 on pBca9145-M10013 (194bp, EcoRI/BamHI = B)
PCR Ovu015/Ovu018 on A+B (505bp, EcoRI/BamHI)
Sub into pBca9145-Bca1144#5 (EcoRI/BamHI, L)
Product is pBca9495KC-M10014 {
Ovu019 SOEing of eCPX
gtcgcTTTAGACTGTTTAGATCCgaagcggtaaccaacag
Ovu020 SOEing of eCPX
GGATCTAAACAGTCTAAAgcgacttctactgtaactgg