BBa_M10024

BBa_M10024 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M10024

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Hank Shih

Date created: 2009-05-08 11:00:00

Date modified: 2015-05-08 01:13:51

{a~Int-native>} Intimin (native)

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_M10024_sequence (Version 1)

Description

TBA

Notes

Construction of {a~Inv-native>} basic part
PCR Bhs001F/Bhs002R on E. coli strain 0157:H7 (146 bp, gp = A)
PCR Bhs002F/Bhs003R on E. coli strain 0157:H7 (1889 bp, gp = B)

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PCR Bhs001F/Bhs003R on A+B (2009 bp, EcoRI/BamHI)
Digest pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L)
Product is pBca9495CA-M10024 {a~Inv-native>}

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Bhs001F Forward EcoRI for {a~Inv-native>} cccaaGAATTCatgAGATCTtaacATGATTACTCATGGTTG
Bhs002F Removing the EcoRI site from {a~Inv-native>} GTTAATCAGAACTCATTTGCAAATGG
Bhs002R Removing the EcoRI site from {a~Inv-native>} CCATTTGCAAATGAGTTCTGATTAAC
Bhs003R Reverse BamHI for {a~Inv-native>} GCAAAggatccGGCCTTGGTTTGATCAAAAAATATAACCGCAC

Source

Genomic sequence of E. coli strain 0157:H7

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M10024/1

Member of these Collections

• iGEM Parts Registry