Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jennifer Brophy
Date created: 2009-04-30 11:00:00
Date modified: 2015-05-08 01:13:51
{˂
CPG_L2!}
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_M10086_sequence (Version 1) |
Description
Circularly permutated OmpG with a backbone opening in loop 2 of the native protein.Circular permutation re-orders the protein so that both termini are exposed to the extra-cellular environment.
CPG_L2 is an outer membrane carrier protein that can be used to display other proteins on the outer membrane. "Display proteins" can be fused to the N-termini of {
Notes
Needed to design appropriate linkers (to fuse the N and C termini of the native protien). Used 6aa linker (CDS: GGATCTAAGTCTCGTCGC) from PMID: 18480093.The opening was created in loop 2 (aa 59-60) because the residues of the mature protein are presumably disordered because they are not visible in electron density maps.
Source
E. coli MG1655 genomic DNA| Sequence Annotation | Location | Component / Role(s) |
| Linker Native N termini Native C termini | 664,681 682,861 1,663 | feature/misc sequence_feature feature/protein CDS feature/protein CDS |