BBa_M10090

BBa_M10090 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M10090
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Douglas Densmore
Date created: 2009-05-03 11:00:00
Date modified: 2015-05-08 01:13:51

{˂
Chal˃
}



    • Details

    Types
    DnaRegion

    Roles
    sequence_feature

    Other

    Sequences BBa_M10090_sequence (Version 1)

    Description

    For background you should read PMID: 15039097. ChaI is a lectin (a sugar-binding protein) that binds to polysialic acid which is the outer shell composition of E. coli expressing the K1 capsule.

    Notes

    Part designed during Bio140L at UC Berkeley during Spring 2009

    Source

    Construction of {} basic part from pBca9145-Jtk2031
    PCR ca998 and Odmd003R on pBca9145-Jtk2031 (510bp, EcoRI/BamHI)
    Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, 3171+910, L)
    Product is pBca9495AK-M10051 {}
    ----------------------------
    ca998 Forward Sequencing of pSB1A2/pSB1A3 gtatcacgaggcagaatttcag
    Odmd003R Reverse Oligo for Chal Lectin w/o Stop Codon ccaaaGGATCCctcgctgtcgtcctctcgta

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_M10090/1
     

    Attachments

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