Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Angela Dixon
Date created: 2010-04-20 11:00:00
Date modified: 2015-05-08 01:13:53
pRS316 plasmid for S. cerevisiae in BioBrick format
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_M11201_sequence (Version 1) |
Description
Modified pRS316 plasmid for S. cerevisiae. Has selection for tryptophan negative, Ampicillin, and LacZ. Can be used to insert BioBricks into S. cerevisiae.Notes
Used mutagenesis to remove EcoRI site at 389???394. Also used mutagenesis to rearrange the EcoRI, XbaI, SpeI, and PstI into BioBrick format. Added the tryptophan negative gene for selection.Source
Comes from the genomic sequence of pRS316| Sequence Annotation | Location | Component / Role(s) |
| EcoRI XbaI SpeI PstI Tryptophan Synthase lacZ Ampicillin Resistance | 3349,3354 3355,3360 3373,3378 3379,3384 523,1783 2913,3486 4645,5503 | feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc CDS feature/protein feature/misc sequence_feature sequence_feature feature/misc |