Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alyssa Calder
Date created: 2010-04-19 11:00:00
Date modified: 2015-05-08 01:13:54
Copper Promoter (CueR regulated) with GFP Reporter
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M11511_sequence (Version 1) |
Description
Promoter sequence with recognition site for the CueR transcription regulating protein. When copper levels in the cell rise CueR activates transcription (1) and produces GFP as a reporter. This is a new Silver-fusion compatible GFP BioBrick part. This particular GFP was previously mutated for improved fluorescence photostability (Crameri, 1996). The excitation and emission wavelengths for this GFP are 395 nm and 509 nm, respectively. That being said, GFP-positive cells emit a bright green fluorescence when exposed to shorter-wavelength UV light, such as on a transilluminator.Notes
This part is a composite of part BBa_K190017 and BBa_K208000Source
For further information and characterization of the CueO promotor see CueO1) K. Yamamoto and A. Ishihama (2005) Transcriptional response of Escherichia coli to external copper, Molecular Microbiology, 56(1), 215???227.
| Access | Instance | Definition |
| public public public public public | BBa_K190017 BBa_B0034 BBa_K208000 BBa_B0010 BBa_B0012 | BBa_K190017 BBa_B0034 BBa_K208000 BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K190017 BBa_B0034 BBa_K208000 BBa_B0010 BBa_B0012 | 1,43 52,63 70,789 798,877 886,926 | BBa_K190017 BBa_B0034 BBa_K208000 BBa_B0010 BBa_B0012 |