Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alyssa Calder
Date created: 2010-04-19 11:00:00
Date modified: 2015-05-08 01:13:54
Zinc Promoter (ZntR regulated) with GFP Reporter
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M11521_sequence (Version 1) |
Description
Promoter sequence with recognition site for ZntR transcriptional regulator protein. ZntR activates transcription when Zn(II) is bound (1) and produces GFP. This is a new Silver-fusion compatible GFP BioBrick part. This particular GFP was previously mutated for improved fluorescence photostability (Crameri, 1996). The excitation and emission wavelengths for this GFP are 395 nm and 509 nm, respectively. That being said, GFP-positive cells emit a bright green fluorescence when exposed to shorter-wavelength UV light, such as on a transilluminator.Notes
Composite part of BBa_K190016 and BBa_K208000Source
1) C.E. Outten, F.W. Outten, T.V. O???Halloran (1999) DNA Distortion Mechanism for Transcriptional Activation by ZntR, a Zn(II)-responsive MerR Homologue in Escherichia coli, The Journal of Biological Chemistry, Vol. 274, No. 53, Issue of December 31, pp. 37517???37524.| Access | Instance | Definition |
| public public public public public | BBa_K190016 BBa_B0034 BBa_K208000 BBa_B0010 BBa_B0012 | BBa_K190016 BBa_B0034 BBa_K208000 BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K190016 BBa_B0034 BBa_K208000 BBa_B0010 BBa_B0012 | 1,69 78,89 96,815 824,903 912,952 | BBa_K190016 BBa_B0034 BBa_K208000 BBa_B0010 BBa_B0012 |