BBa_M11544

BBa_M11544 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M11544
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alyssa Calder
Date created: 2010-04-20 11:00:00
Date modified: 2015-05-08 01:13:54

Fe (iron) Promoter (point mutations)



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_M11544_sequence (Version 1)

Description

This promoter is a mutated version of part BBa_I765000 at all of its PstI restriction sites.

Notes

There were several PstI restriction sites that had to be mutated. The six nucleotide sequence for PstI is ctgcag. We mutated it so that the sequence became ctacag, keeping the appropriate amino acid still in place, but getting rid of the potential for PstI to cut it.

Source

Part BBa_I765000

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M11544/1