BBa_M12072

BBa_M12072 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M12072

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Will Whitney

Date created: 2010-05-11 11:00:00

Date modified: 2015-05-08 01:13:55

CueR internal copper sensor

Details

• Types: DnaRegion
• Roles: engineered_region; Measurement
• Sequences: BBa_M12072_sequence (Version 1)

Description

CueR is a copper-sensitive activator/repressor found in Yersinia pseudotuberculosis which performs a task for copper similar to that performed by merR for mercury. In absence of copper, it represses the transcription of the genes downstream of it (in source, cueTPAD), but when bound to copper it causes underwinding of cueOP DNA and thus promotes RNA polymerase transcription of the region. In the source genome, the following sequences are copper coping mechanisms.

Any genes inserted downstream of cueR will be transcribed only in the presence of copper inside the cell. Usable in E. coli and various other bacteria.

Notes

none

Source

Yersinia pseudotuberculosis genome. http://www.ncbi.nlm.nih.gov/gene/2955378

Information on function from
JL Hobman, J Wilkie, NL Brown. "A design for life: prokaryotic metal-binding MerR family regulators." Biometals, 2005.
http://www.springerlink.com/content/u7732202161l4847/

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• rdf-schema#seeAlso: Q8ZCA8
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M12072/1

Member of these Collections

• iGEM Parts Registry