Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Aaron Winder
Date created: 2011-04-24 11:00:00
Date modified: 2015-05-08 01:13:55
Kill gene intermediate - Holin/Autolysin portion
| Types | DnaRegion |
| Roles | Intermediate engineered_region |
| Sequences | BBa_M1208_sequence (Version 1) |
Description
This part is an intermediate of the completed kill gene, including the anti-holin portion of the construct only. It consists of a constitutive promoter, RBS, antiholin sequence, and terminator. The constitutive promoter has low levels of activity and is meant to neutralize any holin that is incidentally expressed. This low level of activity should not greatly impact the efficacy of the holin when it is to be expressed.Notes
Constitutive promoter should have the correct levels of activity in order to be effective but not to prevent the efficacy of the holins when they are meant to be expressed.Source
BBa_J23116, BBa_J61101, BBa_K112807, BBa_B0010| Access | Instance | Definition |
| public public public public public public | BBa_R0040 BBa_J61102 BBa_E0040 BBa_K112805 BBa_K112806 BBa_B0015 | p(tetR) BBa_J61102 GFP BBa_K112805 BBa_K112806 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 BBa_J61102 BBa_E0040 BBa_K112805 BBa_K112806 BBa_B0015 | 1,54 63,74 81,800 809,1484 1493,2006 2015,2143 | p(tetR) BBa_J61102 GFP BBa_K112805 BBa_K112806 BBa_B0015 |