Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2010-11-24 12:00:00
Date modified: 2015-05-08 01:13:55
PTet : B0034 : GFP in BioScaffold Form, a [0,5
7,2) BioScaffold
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_M12101_sequence (Version 1) |
Description
This is a test part for validation of a BioScaffold project. The part is a green fluorescent protein constitutively expressed by the PTet promoter and the strong Elowitz ribosome binding site.Notes
See documentation about BioScaffold restriction sites- specifically FalI and PsrI- for more information about the requirements for BioScaffold designs.Source
This is a modified version of the BBa_I13522 part and contains a truncated terminator primarily for ease of use with BioScaffold-oriented priming and assembly.| Sequence Annotation | Location | Component / Role(s) |
| Elowitz RBS Tet Promoter GFP Left BioScaffold Padding Right BioScaffold Padding Truncated Terminator FalI FalI | 81,92 19,72 99,818 1,18 860,876 827,876 7,17 859,870 | feature/rbs ribosome_entry_site feature/promoter promoter CDS feature/cds engineered_region feature/BioBrick feature/BioBrick engineered_region sequence_feature feature/misc non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding |