Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Natalie Kuldell
Date created: 2007-02-12 12:00:00
Date modified: 2015-05-08 01:13:55
M13K07 genome
| Types | DnaRegion |
| Roles | engineered_region Project |
| Sequences | BBa_M1307_sequence (Version 1) |
Description
M13 is a filamentous phage that infects E. coli. It packages a single stranded genome of ~6400 bases, encoding 11 ORFs. The M13K07 sequence entered here includes the entire M13 genome as well as a sequence from pACYC177 bearing the p15a bacterial origin of replication and a kanamycin resistance gene. These additional sequences were inserted into the packaging sequence of M13, enabling the phage to propogate as a Kan-selectable plasmid. The modification does attenuate phage production though plaques are still detectable on NEB strain ER2267 (genotype: : F?? proA+B+ lacIq Δ(lacZ)M15 zzf::mini-Tn10 (KanR)/ Δ(argF-lacZ)U169 glnV44 e14-(McrA-) rfbD1? recA1 relA1? endA1 spoT1? thi-1 Δ(mcrC-mrr)114::IS10). In the presence of a second plasmid bearing the M13 packaging sequence and origin, M13K07 serves as a "helper phage," providing the proteins for the phage coat but allowing the alternative plasmid to be preferentially placed in the phage capsid.Notes
noneSource
sequenced in 2002 by L. Panganaban. sequence downloaded from New England Biolabs.| Sequence Annotation | Location | Component / Role(s) |
| gene 2 promoter-g10 rbs-g10 gene 10 promoter g5 rbs-g5 g5 (ORF) BsrGI rbs-g7 promoter-g8 rbs-g9 gene 9 SnaBI rbs-g8 gene VIII promoter-g3 rbs-g3 gene III (ORF) BamHI promoter-g6 promoter-g6 rbs-g6 gene VI promoter-g1 g1 rbs-g11 promoter-g4 PacI rbs-g4 rbs g-4 g4 ApaLI MscI NgoMIV DrdI SgrAI SacII BssSI BstBI AhdI SacI HindIII HindIII BsmBI PvuI EcoNI NruI TliI XhoI Pst1 promoter-g2 SwaI rbs-g2 rbs-g1 AccI HpaI | 1,831 381,428 480,495 496,831 786,835 827,842 843,1106 1021,1025 1092,1107 1155,1201 1190,1205 1206,1304 1268,1273 1285,1300 1301,1522 1500,1547 1563,1578 1579,2853 2220,2224 2716,2764 2716,2764 2840,2855 2856,3194 3086,3132 3196,4242 3900,3915 4055,4103 4131,4138 4204,4219 4204,4219 4220,5500 4743,4748 5080,5085 5613,5617 5763,5765 5867,5871 6030,6035 6158,6162 6518,6520 6824,6834 6951,6956 7402,7406 7402,7407 7504,7508 7524,7526 7611,7612 7865,7870 7922,7926 7922,7926 8079,8083 8188,8235 8202,8209 8252,8267 8252,8267 8353,8358 8669,8669 | CDS feature/protein promoter feature/promoter feature/rbs ribosome_entry_site feature/protein CDS promoter feature/promoter feature/rbs ribosome_entry_site feature/protein CDS sequence_feature feature/misc feature/rbs ribosome_entry_site promoter feature/promoter ribosome_entry_site feature/rbs CDS feature/protein sequence_feature feature/misc ribosome_entry_site feature/rbs CDS feature/protein promoter feature/promoter ribosome_entry_site feature/rbs CDS feature/protein feature/misc sequence_feature feature/promoter promoter promoter feature/promoter feature/rbs ribosome_entry_site feature/protein CDS promoter feature/promoter feature/protein CDS ribosome_entry_site feature/rbs promoter feature/promoter sequence_feature feature/misc feature/rbs ribosome_entry_site feature/rbs ribosome_entry_site CDS feature/protein sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/promoter promoter feature/misc sequence_feature feature/rbs ribosome_entry_site feature/rbs ribosome_entry_site feature/misc sequence_feature sequence_feature feature/misc |