BBa_M1631

BBa_M1631 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M1631
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oumou Diallo
Date created: 2013-04-11 11:00:00
Date modified: 2015-05-08 01:13:57

Mutated lacI 1



    • Details

    Types
    DnaRegion

    Roles
    promoter

    Regulatory

    Sequences BBa_M1631_sequence (Version 1)

    Description

    lacI was mutated by removing the lactose binding site (AA 75,76,79)and replaced by hydrogen binding site (His, His, Cys)

    Notes

    Back translated and optimized to remove standard restriction sites

    Source

    LacI amino acid sequence with H2 binding-related amino acids, as discussed in www.sciencemag.org/cgi/content/321/5888/572/DC1 (GenBank: AAB98710.1)

    Sequence Annotation Location Component / Role(s)
    Codes for H2 binding site
    		223,237
    		feature/mutation sequence_alteration

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_M1631/1
     

    Attachments

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