Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Ying
Date created: 2007-03-06 12:00:00
Date modified: 2015-05-08 01:13:59
M13.1 from HpaI to BamHI, for reals this time!
| Types | DnaRegion |
| Roles | engineered_region Project |
| Sequences | BBa_M31173_sequence (Version 1) |
Description
This is my (new) modification to a section of the M13K07 genome from the unique HpaI site in gene 2 to the BamHI site in gene 3.Notes
My main objective was to move the promoter for g8 closer to its ORF, but in the process I had to unstuff various genetic elements involving g7 and g9.Source
M13K07 bacteriophage genome| Sequence Annotation | Location | Component / Role(s) |
| g2 ORF (incomplete) HpaI (incomplete) g10 promoter g10 RBS g10 ORF g5 promoter g5 RBS g5 ORF g7 RBS g7 ORF MfeI g9 RBS g9 ORF NcoI g8 promoter g8 RBS g8 ORF NheI g3 promoter g3 RBS g3 ORF (incomplete) BamHI | 1,831 1,3 381,428 480,495 496,831 785,835 827,842 843,1106 1092,1107 1108,1209 1210,1215 1216,1231 1232,1330 1331,1336 1337,1383 1384,1399 1400,1621 1647,1652 1599,1646 1668,1683 1684,2330 2325,2330 | feature/cds CDS feature/misc sequence_feature feature/promoter promoter ribosome_entry_site feature/rbs CDS feature/cds promoter feature/promoter feature/rbs ribosome_entry_site feature/cds CDS feature/rbs ribosome_entry_site feature/cds CDS sequence_feature feature/misc feature/rbs ribosome_entry_site CDS feature/cds sequence_feature feature/misc feature/promoter promoter ribosome_entry_site feature/rbs feature/cds CDS feature/misc sequence_feature promoter feature/promoter feature/rbs ribosome_entry_site CDS feature/cds sequence_feature feature/misc |