Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Oh
Date created: 2007-02-27 12:00:00
Date modified: 2015-05-08 01:13:59
M13.1: Refactored M13K07 Phage
| Types | DnaRegion |
| Roles | Project engineered_region |
| Sequences | BBa_M31270_sequence (Version 1) |
Description
The following sequence of DNA is drawn from the M13K07 phage plasmid, specifically the section between the HpaI and BamHI restriction sites. This section incorporates all or parts of seven open reading frames, many of which are overlapping in the wild-type genotype. The result is a synthetic biology-oriented genome that can be better understood and hopefully will be more easily manipulated for bioengineering purposes.Notes
Overlaps were eliminated through direct duplication of appropriate sequences. Homologous recombination possibilities were diminished by silent mutation of the first instance of duplicated codons. About half the duplicated codons were changed in this manner.Source
The source DNA is bases 1-2435 from the M13K07 phage, which is bounded by the HpaI and BamHI restriction sites.| Access | Instance | Definition |
| public public public public public public public public public public public public public public public public public public public | BBa_M31372 BBa_M31380 BBa_M13105 BBa_M13505 BBa_M31375 BBa_M31381 BBa_M13507 BBa_M31377 BBa_M31382 BBa_M13509 BBa_M31379 BBa_M31383 BBa_M31370 BBa_M13508 BBa_M31378 BBa_M31384 BBa_M13103 BBa_M13503 BBa_M31373 | BBa_M31372 BBa_M31380 BBa_M13105 BBa_M13505 BBa_M31375 BBa_M31381 BBa_M13507 BBa_M31377 BBa_M31382 BBa_M13509 BBa_M31379 BBa_M31383 BBa_M31370 BBa_M13508 BBa_M31378 BBa_M31384 BBa_M13103 BBa_M13503 BBa_M31373 |