Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stephanie Nix
Date created: 2007-02-26 12:00:00
Date modified: 2015-05-08 01:14:00
20.109 refactor
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M31415_sequence (Version 1) |
Description
A refactored part of the M13KO7 genome. Separates overlapping parts if possible, and inserts zero cutters in between parts.Notes
Homologous recombination, redesigning existing genes to get rid of existing overlap.Source
M13KO7: 8668-2225| Sequence Annotation | Location | Component / Role(s) |
| mod g2 g10 promoter g10 RBS mod g10 g5 promoter g5 RBS mod g5 BsrGI g7 RBS mod g7 g8 promoter g9 RBS mod g9 SnaBI g8 RBS mod g8 g3 promoter HpaI g3 RBS mod g3 BamHI AatII Acc65I ApaI AscI BclI BmtI BsiWI EcoRI KasI MfeI | 1,834 841,888 895,910 911,1246 1253,1302 1311,1326 1327,1609 1524,1529 1616,1631 1632,1733 1740,1786 1793,1808 1809,1907 1871,1876 1914,1929 1930,2151 2158,2205 1,6 2212,2227 2228,2874 2869,2874 835,840 889,894 1247,1252 1303,1310 1610,1615 1734,1739 1787,1792 1908,1913 2152,2157 2206,2211 | feature/protein CDS feature/promoter promoter ribosome_entry_site feature/rbs CDS feature/protein feature/promoter promoter ribosome_entry_site feature/rbs CDS feature/protein sequence_feature feature/misc feature/rbs ribosome_entry_site feature/protein CDS promoter feature/promoter ribosome_entry_site feature/rbs CDS feature/protein sequence_feature feature/misc ribosome_entry_site feature/rbs CDS feature/protein promoter feature/promoter sequence_feature feature/misc ribosome_entry_site feature/rbs CDS feature/protein sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc |