Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Emmanuel Lorenzo de los Santos
Date created: 2007-02-25 12:00:00
Date modified: 2015-05-08 01:14:00
section of M13.1 (HpaI to BamHI section)
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_M31512_sequence (Version 1) |
Description
We are designing a refactored M13 that we are calling M13.1, we decided to do the refactoring of the genome by sectors bordered by unique restriction sites on the M13K07 genome, this is to ensure that the M13.1 we design is modular enough that we can easily revert to the original section if the refactored section is unsucessful.Notes
none so farSource
Comes from a modified M13K07 genome sequence| Sequence Annotation | Location | Component / Role(s) |
| gene 10 ORF gene 2 ORF (inc) HpaI (inc) gene 5 ORF gene 7 ORF gene 9 ORF gene 8.1 ORF PstI gene 3 ORF (inc) BamHI promoter g5 promoter g10 rbs g10 rbs g5 BsrGI rbs g7 promoter g8.1 rbs g8.1 promoter g3 rbs g3 rbs g9 SnaBI gene 8.2 promoter rbs g8.2 gene 8.2 ORF KpnI EagI ApaI AvrII BspEI BglII NcoI NarI SpeI BclI KasI BsiWI SpeI BclI KasI BsiWI | 496,831 1,831 1,3 900,1163 1191,1292 1355,1453 1481,1702 1368,1373 2133,2779 2774,2779 843,892 381,428 480,495 893,899 1078,1083 1175,1190 1304,1350 1465,1480 2054,2100 2117,2132 1339,1354 1268,1273 1747,1793 1794,1809 1810,2031 832,837 837,842 1164,1169 1169,1174 1293,1298 1298,1303 1454,1459 1459,1464 1703,1708 1708,1713 1714,1719 1719,1724 2032,2037 2037,2042 2043,2048 2048,2053 | CDS feature/protein feature/protein CDS sequence_feature feature/misc feature/protein CDS feature/protein CDS feature/protein CDS feature/protein CDS feature/misc sequence_feature CDS feature/protein sequence_feature feature/misc promoter feature/promoter promoter feature/promoter feature/rbs ribosome_entry_site feature/rbs ribosome_entry_site sequence_feature feature/misc ribosome_entry_site feature/rbs feature/promoter promoter feature/rbs ribosome_entry_site promoter feature/promoter feature/rbs ribosome_entry_site ribosome_entry_site feature/rbs sequence_feature feature/misc feature/promoter promoter feature/rbs ribosome_entry_site CDS feature/protein feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature |