Types | DnaRegion
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Roles | DNA
sequence_feature
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Sequences | BBa_M31537_sequence (Version 1)
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Description
When designing a phage, it is much more practical and effective to think of DNA in functional units than strings of regulatory and coding regions. This part combines the transcriptional and translational regions with the ORF to form a functional (where function is defined as the output of the gene).
Notes
When removed from its place in the genome that has been determined by evolution, we can't be sure the change will be tolerated. There may be cross-talk between copied and separated elements with significant homology. Because we have removed and maintained entire portions of genes in the formation of separate genomic entities, there is also the possibility of expression of these 'partial' genes. I will address this by placing transcriptional terminators and hair pins between genes.
Source
Though the gene and its regulatory sequences exist in nature in M13K07, they do not yet exist in this separate form.