BBa_M33330

BBa_M33330 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M33330

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Frank Li

Date created: 2010-05-31 11:00:00

Date modified: 2015-05-08 01:14:01

RC of atzR CDS

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_M33330_sequence (Version 1)

Description

Pseudomonas sp. ADP digests atrazine under nitrogen starvation conditions. atzABC are constitutively expressed and convert atrazine to cyanuric acid. atzDEF convert cyanuric acid to carbon dioxide and ammonia, which the cell digests. The atzDEF operon is regulated by atzR, a protein that autoregulates its production in the same region. GlnK is produced under nitrogen limitation conditions and induces expression of atzDEF.

This part should be used in conjunction with BBa_M33333, the atzDEF operator region. See related parts BBa_M33331, BBa_M33332, and BBa_M33333.

Notes

We made 4 silent point mutations to make this sequence conform to Assembly Standard 10: 3 for EcoRI and 1 for NotI. We also edited to make TAA the stop codon.

This sequence may not work in E. coli.

Source

From Pseudomonas sp. ADP at http://www.ncbi.nlm.nih.gov/nuccore/13937422, bp 99885..100853. Formerly called orf99.

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M33330/1

Member of these Collections

• iGEM Parts Registry