BBa_M36025

BBa_M36025 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M36025
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jon Rodriguez
Date created: 2011-05-02 11:00:00
Date modified: 2015-05-08 01:14:02

phiX174 gene B - mutated rbs of K



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_M36025_sequence (Version 1)

    Description

    Took the wt gene B from coliphage phiX174, and then mutated it synonymously in order to destroy the overlapping RBS of gene K

    Notes

    Made only synonymous changes, and tried to keep the codon usage bias as similar as possible to wt.

    Source

    wt phiX174, then mutated by Jon Rodriguez and Adrian Sierra

    Sequence Annotation Location Component / Role(s)
    Start Codon
    Stop Codon
    CDS
    a->t
    		1,3
    361,363
    1,363
    354,354
    		start_codon feature/start
    stop_codon feature/stop
    feature/cds CDS
    point_mutation feature/s_mutation

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    rdf-schema#seeAlso
    P03633
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_M36025/1
     

    Attachments

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