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BBa_M36026

BBa_M36026 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M36026

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jon Rodriguez

Date created: 2011-05-02 11:00:00

Date modified: 2015-05-08 01:14:02

phiX174 gene K - mutated rbs and start codon of C

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Details

Types	DnaRegion
Roles	Coding CDS
Sequences	BBa_M36026_sequence (Version 1)

Description

Took the wt gene K from coliphage phiX174, and then mutated it synonymously in order to destroy the RBS and start codon of the overlapping gene C.

Notes

Made only synonymous changes, and tried to keep the codon usage bias as similar as possible to wt, according to this table http://openwetware.org/wiki/Escherichia_coli/Codon_usage

Source

wt phiX174, then mutated by Jon Rodriguez and Adrian Sierra

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start codon stop codon CDS g->c g->c t->c	1,3 169,171 1,171 72,72 75,75 84,84	start_codon feature/start feature/stop stop_codon CDS feature/cds point_mutation feature/s_mutation point_mutation feature/s_mutation point_mutation feature/s_mutation