BBa_M36027

BBa_M36027 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M36027

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jon Rodriguez

Date created: 2011-05-02 11:00:00

Date modified: 2015-05-08 01:14:02

phiX174 gene C - mutated rbs and start codon of D

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_M36027_sequence (Version 1)

Description

Took the wt gene C from coliphage phiX174, and then mutated it synonymously in order to destroy the RBS and start codon of the overlapping gene D.

Notes

Made only synonymous changes, and tried to keep the codon usage bias as similar as possible to wt, according to this table http://openwetware.org/wiki/Escherichia_coli/Codon_usage

Source

wt phiX174, then mutated by Jon Rodriguez and Adrian Sierra

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start codon	1,3	start_codon, feature/start
stop codon	259,261	stop_codon, feature/stop
CDS	1,261	feature/cds, CDS
a->c	246,246	feature/s_mutation, point_mutation
t->c	249,249	feature/s_mutation, point_mutation
a->t	258,258	point_mutation, feature/s_mutation

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• rdf-schema#seeAlso: P69172
• rdf-schema#seeAlso: P69173
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M36027/1

Member of these Collections

• iGEM Parts Registry