Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jon Rodriguez
Date created: 2011-05-02 11:00:00
Date modified: 2015-05-08 01:14:02
phiX174 gene D - mutated rbs and start codon of E, and rbs of J
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_M36028_sequence (Version 1) |
Description
Took the wt gene K from coliphage phiX174, and then mutated it synonymously in order to destroy the RBS and start codon of the overlapping gene E, and the overlapping RBS of gene J.Notes
Made only synonymous changes, and tried to keep the codon usage bias as similar as possible to wt, according to this table http://openwetware.org/wiki/Escherichia_coli/Codon_usageSource
wt phiX174, then mutated by Jon Rodriguez and Adrian Sierra| Sequence Annotation | Location | Component / Role(s) |
| start codon stop codon CDS t->c g->a a->g a->t | 1,3 457,459 1,459 180,180 168,168 447,447 450,450 | start_codon feature/start stop_codon feature/stop CDS feature/cds point_mutation feature/s_mutation point_mutation feature/s_mutation point_mutation feature/s_mutation feature/s_mutation point_mutation |