Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rahul Sastry, Anjan Katta
Date created: 2013-06-09 11:00:00
Date modified: 2015-05-08 01:14:02
Adapted fnr binding site
| Types | DnaRegion |
| Roles | ribosome_entry_site RBS |
| Sequences | BBa_M36068_sequence (Version 1) |
Description
This sequence is an adapted binding site for the FNR (fumarate and nitrate reductase) protein. FNR is a transcriptional activator that, when functional, should bind to this site and recruit RNA polymerase.Notes
The size of the sequence and number of included binding sites had to be greatly reduced in order to minimize repeats and facilitate synthesis.Source
The DNA sequence recognized by FNR consists of an inverted repeat with a consensus sequence of TTGATnnnnATCAA. To create an appropriately sized and spaced eukaryotic promoter that would be recognized by FNR, we adopted the GAL4 Upstream Activating Sequences (UAS) from the Invitrogen pGene/V5-His A plasmid. The 6 GAL4 binding sites were replaced with 3 FNR binding sites (to minimize repeats and, therefore, simplify DNA production), and the sequence was truncated about 30 bp after the included TATA box.| Sequence Annotation | Location | Component / Role(s) |
| fnr binding site fnr binding site fbr binding site tata box | 24,37 55,68 40,53 81,93 | feature/promoter promoter promoter feature/promoter promoter feature/promoter feature/promoter promoter |