Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joseph Shih
Date created: 2013-05-07 11:00:00
Date modified: 2015-05-08 01:14:03
S. cerevisiae actuator test rig
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_M36092_sequence (Version 1) |
Description
S. cerevisae test vector for actuators. Has a Gal1 promoter which is galactose inducible and glucose repressible. Comes with high copy number origin, kanamycin resistance, and leucine yeast selection; can replace with low copy number origin if ordering from DNA 2.0.Notes
Has S. cerevisiae specific LEU selection and 2 um origin of replication allowing for 50-100 copies of the plasmid in yeast cells.Source
Vector is based off of pJ1201-03c plasmid developed by DNA2.0.| Sequence Annotation | Location | Component / Role(s) |
| Pamp KanR pUCori Gal1 promoter student actuators go here Pleu Leu Leu polyA 2um ori | 1,128 129,938 939,1742 1757,2171 2173,2883 2899,3398 3399,4493 4494,4593 4598,6043 | promoter feature/promoter CDS feature/cds sequence_feature feature/misc promoter feature/promoter sequence_feature feature/misc promoter feature/promoter feature/cds CDS feature/polya polyA_site feature/misc sequence_feature |