BBa_M36094

BBa_M36094 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M36094

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Joseph Shih

Date created: 2013-04-22 11:00:00

Date modified: 2015-05-08 01:14:03

S. elongatus cyanobacteria actuator test rig

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_M36094_sequence (Version 1)

Description

S. elongatus test vector for sensors. Has a cyanocitrus YFP coding sequence as a reporter. Comes with high copy number origin and kanamycin resistance.

Notes

Source plasmid is pNS2-YFP. pNS2-YFP is a plasmid made by Rachel Hood from Dr. Dave Savage's lab.

Source

Redesigned by the BIOE44 teaching staff from pNS2-YFP.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
E. coli ORI	1,861	sequence_feature, feature/misc
NS2 site #1	1072,2555	feature/conserved, conserved_region
lacI repressor	2786,3868	feature/protein, CDS
Lac promoter	4100,4129	feature/promoter, promoter
student actuators go here	4130,4171	feature/misc, sequence_feature
rrnB transcription terminator	4172,4740	stem_loop, feature/stem_loop
KanR cassette	4751,6002	CDS, feature/protein
NS2 site #2	6008,6740	conserved_region, feature/conserved

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M36094/1

Member of these Collections

• //plasmidbackbone
• iGEM Parts Registry
• igem_plasmid_vectors