BBa_M36095

BBa_M36095 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M36095

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Joseph Shih

Date created: 2013-04-22 11:00:00

Date modified: 2015-05-08 01:14:03

S. elongatus cyanobacteria sensor test rig

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_M36095_sequence (Version 1)

Description

S. elongatus test vector for sensors. Has a cyanocitrus YFP coding sequence as a reporter. Comes with high copy number origin and kanamycin resistance

Notes

Redesigned by the BIOE44 teaching staff from pNS2-YFP.

Source

Source plasmid is pNS2-YFP. pNS2-YFP is a plasmid made by Rachel Hood from Dr. Dave Savage's lab.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
NS2 site #1	1,1484	conserved_region, feature/conserved
student sensors go here	1715,1751	sequence_feature, feature/misc
RBS	1752,1766	feature/rbs, ribosome_entry_site
cyanocitrus YFP	1767,2482	feature/cds, CDS
rrnB transcription terminator	2496,3064	stem_loop, feature/stem_loop
KanR cassette	3075,4326	feature/protein, CDS
NS2 site #2	4332,5064	conserved_region, feature/conserved
E. coli ORI	7982,8842	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M36095/1

Member of these Collections

• //plasmidbackbone
• iGEM Parts Registry
• igem_plasmid_vectors