Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joseph Shih
Date created: 2013-04-24 11:00:00
Date modified: 2015-05-08 01:14:03
E. coli actuator test rig
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_M36098_sequence (Version 1) |
Description
E. coli test vector for actuators. Has a rhamnose inducible, glucose repressible promoter. Comes with high copy number origin and ampicillin resistance; can replace with low copy number origin and kanamycin resistance if ordering from DNA 2.0. Vector is licensed to DNA2.0 by Xbrane.Notes
DNA2.0 licenses this plasmid from Xbrane and charges for its use.Source
Xbrane is has a patent on this plasmid, Patent Number: US008138324.| Sequence Annotation | Location | Component / Role(s) |
| rhaR and rhaS regulatory proteins rhaBAD promoter Student actuators go here terminator and pUCori Pamp ampR | 1,1962 1963,2038 2039,2094 2095,3272 3273,3400 3401,4261 | CDS feature/protein promoter feature/promoter sequence_feature feature/misc feature/misc sequence_feature feature/promoter promoter feature/cds CDS |