BBa_M36101

BBa_M36101 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M36101

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andee Wallace, Taylor Nguyen, Chad Viergever

Date created: 2011-05-02 11:00:00

Date modified: 2015-05-08 01:14:03

Promoter, HY5 regulated (2 HY5 binding sites)

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_M36101_sequence (Version 1)

Description

Constitutive promoter with two HY5 binding sites; one bs between -35 and -10, and one between -10 and 0. Binding sites will act as inhibitors for constitutive promoter function.

Notes

Chose to insert 2 binding sites at specific locations in the promoter.

Source

Modified J23100 promoter sequence.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-35	1,6	operator, feature/operator
-10	24,29	feature/operator, operator
HY5	11,15	feature/binding, non_covalent_binding_site
HY5	30,35	feature/binding, non_covalent_binding_site

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M36101/1

Member of these Collections

• iGEM Parts Registry