BBa_M36120

BBa_M36120 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M36120

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alaina Shumate

Date created: 2013-10-24 11:00:00

Date modified: 2015-05-08 01:14:03

5' Bicistronic UTR (strong) contains ATG start Codon.

Details

• Types: DnaRegion
• Roles: RBS; ribosome_entry_site
• Sequences: BBa_M36120_sequence (Version 1)

Description

Used in place of an RBS. This device contains two RBS's to provide more reliability in gene selection. Place upsteam of your gene of interest in an actuator design

Notes

We had to consider the strength in the design of this sequence. Because our protein ( human myoglobin) is not shown to be toxic to e. coli, we want in to be expressed in high numbers and thus we chose a strong BCD.

Source

This is based on research done in the paper "Precise and reliable gene expression via standard transcription and translation initiation elements."

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
misc	1,88	sequence_feature, feature/misc

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M36120/1

Member of these Collections

• iGEM Parts Registry