Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Hart, Gustavo Garcia, Raymond Serrano
Date created: 2015-11-05 12:00:00
Date modified: 2015-11-05 09:22:52
Glucose-6-Phosphate Isomerase for manipulation of glycolysis in yeast
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_M36124_sequence (Version 1) |
Description
Glucose-6-phosphate isomerase (Gis a naturally produced enzyme in most organisms that use glycolysis. It catalyzes the second step of the glycogenic cycle, which is the transformation of glucose-6-phosphate to fructose-6-phosphate. Overexpression of this enzyme in a vector with a strong constitutive promoter could lead to interesting metabolic effects and potentially increase the tolerance of the host organism to high concentrations of glucose.Notes
We edited the genetic sequence to remove repeats greater than 10 base pairs in length, so that it could be synthesized and replicated easily. All mutations introduced were silent to maintain an identical function of the wild type protein. This was to allow better comparison of the effects of overexpression.Source
The part was derived from a uniprot sequence of S cerevisiae (baker's yeast), strain ATCC 204508 / S288c.| Sequence Annotation | Location | Component / Role(s) |
| Silent mutation T to C Silent mutation A to G Silent mutation C to T Silent mutation T to C Silent mutation A to G | 154,154 493,493 787,788 1543,1543 1663,1663 | feature/s_mutation point_mutation point_mutation feature/s_mutation point_mutation feature/s_mutation feature/s_mutation point_mutation feature/s_mutation point_mutation |