Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vir Choksi, Daniel McHugh
Date created: 2012-05-31 11:00:00
Date modified: 2015-05-08 01:14:03
2D inducible RNA scaffold
| Types | DnaRegion |
| Roles | mature_transcript_region RNA |
| Sequences | BBa_M36132_sequence (Version 1) |
Description
Self-assembles into a two-dimensional RNA scaffold with binding domains for MS2 and PP7 proteins. When presented with theophylline, the ribozyme should cleave the scaffold sequences, releasing the aptamers from the existing scaffold and preventing other scaffolds from forming.Notes
The riboswitch was inserted into the scaffold sequence directly upstream of the aptamer. Spacers were used to prevent folding issues with the scaffold and ribozyme components of the transcripts.Source
RNA scaffold comes from Delebecque et al., Science 2011Ribozyme switch comes from Win & Smolke, PNAS 2007
| Access | Instance | Definition |
| public public public public public public public public public | BBa_M36128 BBa_M36129 BBa_M36135 BBa_B0040 BBa_M36136 BBa_M36130 BBa_M36127 BBa_M36126 BBa_M36135 | BBa_M36128 BBa_M36129 BBa_M36135 spacer BBa_M36136 BBa_M36130 BBa_M36127 BBa_M36126 BBa_M36135 |