Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel McHugh
Date created: 2012-05-31 11:00:00
Date modified: 2015-05-08 01:14:03
1D inducible RNA scaffold
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_M36134_sequence (Version 1) |
Description
Self-assembles into a one-dimensional RNA scaffold with binding domains for the MS2 bacteriophage coat protein. When presented with theophylline, the ribozyme should cleave the scaffold sequences, releasing the aptamers from the existing scaffold and preventing other scaffolds from forming.Notes
The riboswitch was inserted into the scaffold sequence directly upstream of the aptamer. The PP7 aptamer was omitted to decrease the length of sequence not involved in scaffold folding. Spacers were used to prevent folding issues with the scaffold and ribozyme components of the transcripts.Source
C. J. Delebecque, A. B. Lindner, P. A. Silver, F. A. Aldaye, ???Organization of intracellular reactions with rationally designed RNA assemblies??? Science 333, 470-474 (2011).M. N. Win, C. D. Smolke, ???A modular and extensible RNA-based gene-regulatory platform for engineering cellular function??? PNAS 36, 14283-14288 (2007).
| Access | Instance | Definition |
| public public public public | BBa_M36125 BBa_M36126 BBa_M36129 BBa_M36135 | BBa_M36125 BBa_M36126 BBa_M36129 BBa_M36135 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M36125 BBa_M36126 BBa_M36129 BBa_M36135 | 1,56 65,184 193,228 237,275 | BBa_M36125 BBa_M36126 BBa_M36129 BBa_M36135 |