BBa_M36146

BBa_M36146 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M36146
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jane Hae Soo Shin
Date created: 2012-06-05 11:00:00
Date modified: 2015-05-08 01:14:03

Cold-inducible promoter for HeLa cells



    • Details

    Types
    DnaRegion

    Roles
    Regulatory

    promoter

    Sequences BBa_M36146_sequence (Version 1)

    Description

    This promoter is up-regulated by the lowering of the temperature to 33 degree Celsius. It has been discovered that the transcription factors for this promoter are NF-kB and SP1, which are endogenous to HeLa cells. This promoter can be used in temperature-varying experiments.

    Notes

    Since the promoter was found in CHO cells first, we used BLAST site to find a similar genetic sequence in HeLa cells.

    Source

    The sequence has been BLASTed to HeLa cells because it was originally found in CHO cells.
    http://ukpmc.ac.uk/articles/PMC3118111/pdf/1472-6750-11-51.pdf

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_M36146/1
     

    Attachments

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