BBa_M36150

BBa_M36150 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M36150
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Monica Chin
Date created: 2014-10-22 11:00:00
Date modified: 2015-05-08 01:14:03

nifRLA promoter



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_M36150_sequence (Version 1)

Description

The nifRLA operon regulates the expression of the nitrogen fixation (nif) genes. NifA activates transcription of nif genes by the alternative form of RNA-polymerase, s54-holoenzyme. NifL is a negative regulatory gene which inhibits the activation of other nif genes by nifA protein. NifR is a repressor binding site, between the promoter of the nifRLA operon and the nifL gene.

Notes

We selected the genomic region upstream of the nifL repressor but following the nifR repressor binding site. This is the general region where the nifRLA promoter is found.

Source

The nifRLA promoter sequence was determined from the nif gene cluster sequence for Azotobacter vinelandii. It is found upstream of the nifL repressor.

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M36150/1