Types | DnaRegion
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Roles | promoter
Regulatory
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Sequences | BBa_M36150_sequence (Version 1)
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Description
The nifRLA operon regulates the expression of the nitrogen fixation (nif) genes. NifA activates transcription of nif genes by the alternative form of RNA-polymerase, s54-holoenzyme. NifL is a negative regulatory gene which inhibits the activation of other nif genes by nifA protein. NifR is a repressor binding site, between the promoter of the nifRLA operon and the nifL gene.
Notes
We selected the genomic region upstream of the nifL repressor but following the nifR repressor binding site. This is the general region where the nifRLA promoter is found.
Source
The nifRLA promoter sequence was determined from the nif gene cluster sequence for Azotobacter vinelandii. It is found upstream of the nifL repressor.