Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sohaib Shaikh and Alfonso Ocampo
Date created: 2014-10-22 11:00:00
Date modified: 2015-05-08 01:14:03
E.coli Temperature Shock Sensor/Actuator
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M36158_sequence (Version 1) |
Description
The structure of this part is the cold shock promoter (BBa_M36156), followed by a ribosome binding site (BBa_B0034), meffBlue (BBa_M361567), a transcriptional terminator (BBa_B1006), and finally a heat shock promoter (BBa_K338001). This temperature shock sensor/actuator produces Comet, a green fluorescent protein, or meffBlue, a blue chromoprotein, under heat shock or cold shock, respectively. MeffBlue, part BBa_B0034, is placed downstream of the cold shock promoter, part BBa_M36156, to induce the synthesis of this blue protein under cold conditions. Although not included in this DNA design, if placed carefully, this DNA design can be inserted in the DNA backbone of E.coli, that already contains Comet, upstream of which contains a ribosome binding site such that transcription of Comet can be activated by the heat shock promoter, part BBa_K338001.Notes
Since we wanted to design E.coli that would produce color under cold shock and heat shock, the this part was designed such that after the heat shock promoter, the gene for Comet would be placed. This is only achievable if the DNA backbone of E.coli contains Comet.Source
See subparts below for part sources.| Access | Instance | Definition |
| public public public public public | BBa_M36156 BBa_B0034 BBa_M36157 BBa_B1006 BBa_K338001 | BBa_M36156 BBa_B0034 BBa_M36157 BBa_B1006 HSP |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M36156 BBa_B0034 BBa_M36157 BBa_B1006 BBa_K338001 | 1,87 96,107 114,782 791,829 838,935 | BBa_M36156 BBa_B0034 BBa_M36157 BBa_B1006 HSP |