Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Luke Murphy, Julia Duncan, Bruce Tiu
Date created: 2015-10-21 11:00:00
Date modified: 2015-12-03 05:10:55
RhNUDX1 - Rose Scent (Geraniol) Precursor Catalyzing Gene
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_M36162_sequence (Version 1) |
Description
Coding sequence for the enzyme RhNUDX1. This protein catalyzes the removal of a phosphate group from geranyl diphosphate to produce geranyl monophosphate (phosphatase is required to convert geranyl monophospate to geraniol, the actual scented compound).Notes
This DNA sequence was created by converting the amino acid sequence of RhNUDX1 using the Genedesigner 2.0. We used the IDT Codon Optimizer tool to improve compatibility with E. coli, altering base pairs to preserve amino acid sequence. To remove restriction site problems with BsaI, we modified nucleotide 12 (G -> A) and nucleotide 33 (G -> A).Source
Sequence found by converting amino acid sequence of RhNUDX1 protein native to Rosa x hybrida roses (Papa Meilland breed).| Sequence Annotation | Location | Component / Role(s) |
| Replace G with A Replace G with A Added TAG | 12,12 33,33 451,453 | feature/misc sequence_feature sequence_feature feature/misc feature/stop stop_codon |