| Types | DnaRegion
|
| Roles | Regulatory
promoter
|
| Sequences | BBa_M36177_sequence (Version 1)
|
Description
pH sensor, active within range 5.5-7.0. Our part is based on the acid inducible promoter region of a rcFB gene (procuding rcFB protein) in Lactococcus lactis. Ou assumptions are as follows: (1) generally Lactococcus lactis genes will work in E. coli, (2) all transcription factors specific to this rcFB gene are endogenous to E. coli, (3) there is no additional layer of feedback controlling the rcFB gene, (4) the rcFB gene is more responsive to lactic acid/lactate than carbohydrates (to which it also reacts), (5) base level of transcription is low in comparison to when gene is turned on.
Notes
We were limited by the following restrictions (1) 200-1500 bp size limit, (2) no Bbsl, Bsal, or BsmBI sites, (3) no repeats of length greater than 5 base pairs.
Source
rcFB transcriptional regulator in Lactococcus lactis genome.
