Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Aaron Thayer
Date created: 2012-05-02 11:00:00
Date modified: 2015-05-08 01:14:03
Ligninase Coding Region
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_M36188_sequence (Version 1) |
Description
This coding region has been created from Cullen et al "Nucleotide sequence of a ligninase gene from Phanerochaete chrysosporium." The sequence was modified so that all introns were removed and potential looping eliminated. Additionally, the amino acids have been chosen such that the tRNA ratios for e. coli has been optimized. Minimizing loops was done using Gene Designer and checking for tRNA in e.coli was accomplished using Optimizer.The coding sequence is given seen here is not identical to the sequence given by Cullen et al. However, this sequence has been checked against the Blast database and been found to be highly homologous to multiple Ligninase Peroxidase Regions. GIven here is the Amino Acid Sequence.
Notes
This enzyme works at pH 4.5 as found here: http://www.springerlink.com/content/7616k56005852420/fulltext.pdf?MUD=MPSanroman et al. "Optimum stability conditions of pH and temperature for ligninaseand manganese-dependent peroxidase from Phanerochaete chrysosporium. Application to in vitro decolorization of Poly R-478 by MnP." World Journal of Microbiology and Biotechnology. 22: 607-612. 2006.