BBa_M36263

BBa_M36263 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M36263

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Diana Gong

Date created: 2014-10-22 11:00:00

Date modified: 2015-05-08 01:14:04

LasI optimized for E. coli

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_M36263_sequence (Version 1)

Description

Quorum sensing is a system bacteria use to coordinate behaviors that correlate to cell density. Each species of bacteria produces and senses a unique, diffusable small molecule. This allows bacteria to "count" how many cells of their species are in the vicinity. Pseudomonas Aeruginosa, an opportunistic human pathogen associated with cystic fibrosis, uses two quorum sensing systems, with one of them being LasI/LasR. LasI produces N-(3-oxododecanoyl)-homoserine lactone, and LasR detects it.

This part begins with a start codon and ends with the stop codon for the LasI gene.

Notes

N/A

Source

Sequence originally obtained from: http://www.pseudomonas.com/getAnnotation.do?locusID=PA1432
The sequence was optimized for E. coli by DNA 2.0.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start Codon	1,3	start_codon, feature/start
Stop Codon	604,606	feature/stop, stop_codon
LasI	1,606	CDS, feature/cds

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• rdf-schema#seeAlso: P33883
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M36263/1

Member of these Collections

• iGEM Parts Registry