BBa_M36264

BBa_M36264 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M36264

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Diana Gong

Date created: 2014-10-22 11:00:00

Date modified: 2015-05-08 01:14:04

LasR optimized for E. coli

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_M36264_sequence (Version 1)

Description

Quorum sensing is a system bacteria use to coordinate behaviors that correlate to cell density. Each species of bacteria produces and senses a unique, diffusable small molecule. This allows bacteria to "count" how many cells of their species are in the vicinity. Pseudomonas Aeruginosa, an opportunistic human pathogen associated with cystic fibrosis, uses two quorum sensing systems, with one of them being LasI/LasR. LasR binds to N-(3-oxododecanoyl)-homoserine lactone, which is produced by LasI, to form a complex that regulates quorum sensing-related genes.

This part begins with a start codon and ends with the stop codon for the LasR gene.

Notes

N/A

Source

The original LasR sequence was obtained from: http://www.uniprot.org/uniprot/P25084

This sequence was optimized for E. coli and our composite part by DNA 2.0.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start Codon	1,3	start_codon, feature/start
Stop Codon	718,720	stop_codon, feature/stop
LasR	1,720	CDS, feature/cds

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M36264/1

Member of these Collections

• iGEM Parts Registry