BBa_M36282

BBa_M36282 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M36282
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sunil Bodapati, Julia Joung, Daniel Esquivel-Reynoso
Date created: 2011-11-29 12:00:00
Date modified: 2015-05-08 01:14:04

5' Bicistronic UTR, includes ATG start codon



Types
DnaRegion

Roles
RBS

ribosome_entry_site

Sequences BBa_M36282_sequence (Version 1)

Description

This sequence contains an RBS followed by the sequence TAATG. The parts number is BBa_M36000 (PartsRegistry). The first RBS sequence is strong, while the second one is weak. This stops the first polymerase with the stop codon TAA, but allows for a second polymerase to bind to the start codon ATG(BIOFAB). This sequence prevents a hairpin loop from being formed with another part of the sequence, which would decrease the amount of translation. The bicistronic sequence ends with a start codon ATG.

Notes

no

Source

BioFab: apFAB563

Sequence Annotation Location Component / Role(s)
Start Codon
86,88
start_codon feature/start
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M36282/1