BBa_M36282

BBa_M36282 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M36282

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sunil Bodapati, Julia Joung, Daniel Esquivel-Reynoso

Date created: 2011-11-29 12:00:00

Date modified: 2015-05-08 01:14:04

5' Bicistronic UTR, includes ATG start codon

Details

• Types: DnaRegion
• Roles: RBS; ribosome_entry_site
• Sequences: BBa_M36282_sequence (Version 1)

Description

This sequence contains an RBS followed by the sequence TAATG. The parts number is BBa_M36000 (PartsRegistry). The first RBS sequence is strong, while the second one is weak. This stops the first polymerase with the stop codon TAA, but allows for a second polymerase to bind to the start codon ATG(BIOFAB). This sequence prevents a hairpin loop from being formed with another part of the sequence, which would decrease the amount of translation. The bicistronic sequence ends with a start codon ATG.

Notes

no

Source

BioFab: apFAB563

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start Codon	86,88	start_codon, feature/start

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M36282/1

Member of these Collections

• iGEM Parts Registry