Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dominique Dabija, Christopher Jackson, Debha Amatya
Date created: 2011-12-06 12:00:00
Date modified: 2015-05-08 01:14:04
Spider Silk GFP Actuator
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M36295_sequence (Version 1) |
Description
This is the full sequence for the spider silk GFP actuator. It was the positive control to test if the FOS and JUN sequences worked. It includes the bicistonic sequence, FOS, a linker, GFP, another linker, JUN, a stop codon, and a transcription terminator. It is triggered by PoPS.Notes
The overall design had a 3000 base pair limit. FOS and JUN leucine zippers were used to covalently bond GFP proteins to create a larger strand.Source
RBS Leader and ATG came from BioFAB. Terminator comes from BioFAB. FOS-JUN leucine zipper sequences were taken from O'Shea et al. They were also codon optimized for E.coli.| Access | Instance | Definition |
| public public public public public public public public | BBa_M36293 BBa_M36501 BBa_K133132 BBa_E0040 BBa_K133132 BBa_M36502 BBa_M36296 BBa_M36292 | BCRBS Fos linker GFP linker Jun halt BBa_M36292 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M36293 BBa_M36501 BBa_K133132 BBa_E0040 BBa_K133132 BBa_M36502 BBa_M36296 BBa_M36292 | 1,88 97,216 225,248 255,974 983,1006 1015,1134 1143,1145 1154,1235 | BCRBS Fos linker GFP linker Jun halt BBa_M36292 |