Nsense

BBa_M36356 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M36356
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ana Ordonez, Chris Harrell
Date created: 2014-10-22 11:00:00
Date modified: 2015-05-08 01:14:04

A sensor that activates in the presence of nitrate in anaerobic conditions in E. coli.



    • Details

    Types
    DnaRegion

    Roles
    promoter

    Regulatory

    Sequences BBa_M36356_sequence (Version 1)

    Description

    This part will used along with a Green fluorescent actuator to test for the presence of nitrate.

    Notes

    We had to deal with finding the appropriate binding areas for the promote without including any of the sequence that codes for proteins.

    Source

    The source for this part comes from the NIR promoter region in E. coli plasmid.

    Sequence Annotation Location Component / Role(s)
    FNR
    NarL/P
    IHF II
    IHF I
    FIS I
    Transcription Start Site
    		103,116
    74,88
    22,48
    49,75
    2,16
    150,150
    		non_covalent_binding_site feature/binding
    feature/binding non_covalent_binding_site
    feature/binding non_covalent_binding_site
    feature/binding non_covalent_binding_site
    non_covalent_binding_site feature/binding
    sequence_feature feature/misc

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_M36356/1
     

    Attachments

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