BBa_M36362

BBa_M36362 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M36362

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Will Connors, Ethan Li, and Shirbi Ish-Shalom

Date created: 2013-10-24 11:00:00

Date modified: 2015-05-08 01:14:04

DbjA Haloalkane Dehalogenase

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_M36362_sequence (Version 1)

Description

This part encodes an enzyme with dehalogenase activity for haloalkane substrates. DbjA catalyzes the cleavage of carbon-halogen bonds to form a primary alcohol and a halide. DbjA generally has higher overall activity than other haloalkane dehalogenases. Unlike most other known haloalkane dehalogenases, DbjA has very high activity on 2-bromo-1-chloropropane and chlorocyclopentane, and it has significant activity towards 1,2-dichloropropane (http://www.biochemj.org/bj/435/0345/bj4350345.htm).

ATG and TAA stop codons are included, so this part can just be flanked by a 5' UTR translation initiator and a 3' transcription terminator for use.

Notes

The amino acid sequence was backtranslated through Gene Designer's genetic algorithm (Maximum Generations: 100; Maximum Mutation Rate: 0.05; Minimum Best Individual Selection Rate: 0.9; Population Size: 50; Number of Progeny: 25) into a codon set optimized for Escherichia coli K12. This algorithm was given additional parameters to avoid BbsI, BsmBI, and BsaI restriction sites (at a weight of 1), hairpins (at a weight of 1), and repeats of at least 10 bp (at a weight of 1). A TAA stop codon was then added at the end of the resulting DNA sequence.

Source

This part was sourced from the genomic sequence of the soil bacterium Bradyrhizobium japonicum USDA110, at Accession NP_767727 and Gene ID 27376198. No mutations were made.

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• rdf-schema#seeAlso: P59337
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M36362/1

Member of these Collections

• iGEM Parts Registry