BBa_M36405

BBa_M36405 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M36405
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Justo A. Caballero, Shamik Mascharak and Sibel Sayiner
Date created: 2012-12-05 12:00:00
Date modified: 2015-05-08 01:14:04

Bicistronic RBS (medium) pFAB1646



Types
DnaRegion

Roles
ribosome_entry_site

RBS

Sequences BBa_M36405_sequence (Version 1)

Description

This is a bicistronic RBS, which consists of a constitutive RBS, and a leader sequence that includes the second RBS. It uses bicistronic junction architecture to tightly control translation. It contains two ribosome binding sites: one controls the gene of interest, and the another synthesizes a short leader protein. The latter bicistronic's translation prevents the former RBS from forming a secondary structure with the gene of interest. This ensures a more consistent expression level of your gene of interest.

Notes

This RBS is of medium strength.

Source

More information can be found at BIOFAB.org.

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/myers
 
synbiohub#ownedBy
user/james
 
synbiohub#topLevel
BBa_M36405/1