Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dominique Dabija, Christopher Jackson, Debha Amatya
Date created: 2011-12-03 12:00:00
Date modified: 2015-05-08 01:14:05
Spider Silk Hexamer
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_M36500_sequence (Version 1) |
Description
This is a spider silk hexamer protein. The monomer amino acid sequence was taken from Xia et al. and codon optimized for expression in E. coli. Repeats above 10 base pairs were also minimized to allow for ease of synthesis.Notes
Needed to minimize repeats and allow for ideal mRNA forming without too many loops while still maintaining codon optimization for E. coli.Source
The monomer amino acid sequence was taken from Xia, X.-X., Z.-G. Qian, C. S. Ki, Y. H. Park, D. L. Kaplan, and S. Y. Lee. "Native-sized Recombinant Spider Silk Protein Produced in Metabolically Engineered Escherichia Coli Results in a Strong Fiber." Proceedings of the National Academy of Sciences 107.32 (2010): 14059-4063. Print.| Sequence Annotation | Location | Component / Role(s) |
| monomer 1 monomer 2 monomer 3 monomer 4 monomer 5 monomer 6 | 1,105 106,210 211,315 316,420 421,525 526,630 | sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc |