Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Valerie Wesley Scott
Date created: 2015-10-21 11:00:00
Date modified: 2015-12-03 07:25:47
In the presence of ATC, produces lacI
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_M36528_sequence (Version 1) |
Description
When this part is put into a yeast cell that has TetR, the TetR will bind to the operator and prevent gene expression. By adding ATC, gene expression will be allowed to proceed and LacI protein will be made.Notes
Ensured codons were optimized for yeast. Short random (10 bp) region RBS and the actual gene put in. Eliminated restriction enzyme sites (Bbs1, Bsa1,BsmB1).Source
LacI Gene: Part:BBa_K642001:Design (parts.igem.org)| Access | Instance | Definition |
| public public public | BBa_R0040 BBa_M36526 BBa_M36525 | p(tetR) BBa_M36526 BBa_M36525 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 BBa_M36526 BBa_M36525 | 1,54 63,74 81,1163 | p(tetR) BBa_M36526 BBa_M36525 |